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Create a flow chart (for your poster) of the steps you go through, and show the positions of the 2 sgRNAs in the sequences. Indicate where the PAM sequences are, and where the complementary sequence of the sgRNA.
Indicate schematically where you expect mutations.
We start from the same gene as J. Chandrasekaran et al. (See part 2), namely the gene eukaryotic translation initiation factor 4Ei, but in tomato.
Find the DNA sequence of this gene in tomato. You can use info on:
https://www.ebi.ac.uk/ena/data/view/AY723733
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