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Obtaining a fully complete and error-free genome sequence is virtually impossible, and even a good approximation is often a complicated and costly endeavour. It is therefore wise to select an approach that optimizes those aspects of the genome sequence that are most important for the success of a given project. One should realize that obtaining a genome sequence by itself is seldom the goal, but instead is a mean to achieve a goal. In this seminar we will investigate the currently leading sequencing, assembly and annotation methods, examine their benefits and drawbacks, and explore possibilities for their combination.
Content:
(1) Basics, performed individually. Reading of the review A field guide to whole-genome sequencing, assembly and annotation, Ekblom et al., 2014.https://doi.org/10.1111/eva.12178 and answering of the accompanying general questions about genome sequencing, assembly and gene prediction. Some of the questions require a little extra information outside of the indicated review, which can be obtained by searching the internet (e.g. through Google).
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