Group 3 Ordering techniques for whole genome sequences

When reading the information try to answer and discuss the following questions. Use these questions as a guideline to select information for your presentation.

a)    What is the main new genome sequence mapping platform?

b)    How are markers identified with this mapping platform?

c)     How is the order of short sequences determined by the Bio Nano platform?

d)    How does linkage mapping work? Name a key advantage of linkage mapping.

e)    Name three disadvantages of traditional linkage mapping.

f)     Why are ordering techniques for genome sequences especially important for plants?

g)    How is a misassembly in a genome sequence (inversion, chimerism) detected with optical mapping?

h)    How is a misassembly in a genome sequence (inversion, chimerism) detected by linkage mapping?

Optical mapping in plant comparative genomics, Tang et al., 2015.

Using linkage maps to correct and scaffold de novo genome assemblies, methods, challenges and computational tools, Fierst, 2015.

Extra:

Linkage mapping: https://en.wikipedia.org/wiki/Genetic_linkage

Optical mapping: www.bionanogenomics.com