Group 1 Sequence confirmation and Short-read NGS

When reading the information try to answer and discuss the following questions. Use these questions as a guideline to select information for your presentation.

a)    How are basepairs identified in nowadays First generation (Sanger) sequencing?

b)    How is the order of the basepairs determined in First generation sequencing?

c)     How are basepairs identified in Next generation sequencing (NGS)?

d)    How is the order of the basepairs determined in Next generation sequencing?

e)    What are the main DNA preparation steps for First generation sequencing?

f)     What are the main DNA preparation steps for Next generation sequencing?

g)    What are the differences between First and Next generation sequencing?

h)    What is the main NGS sequencing platform?

i)      List three advantages of NGS in comparison to First generation sequencing.

j)     List three disadvantages of NGS (in general).

k)    List three other applications of NGS in addition to sequencing for de novo genome assembly.

DNA sequencing at 40, past present and future, Shendure et al., 2017.

Illumina_An introduction to next generation sequencing technology.

Extra:

Sanger: https://en.wikipedia.org/wiki/Sanger_sequencing

Illumina: https://www.illumina.com/systems/sequencing-platforms.html