Group 5 Genome annotation

a)    What is structural annotation?

b)    What is ‘ab initio’ discovery?

c)     Why is masking important?

d)    What is functional annotation?

e)    What is an important first step for functional annotation?

f)     Why are domain-based annotation tools often more accurate than similarity-based tools?

g)    What happens if your structural annotation is poor, because the genome sequence is of poor quality?

h)    What is the most widely used functional annotation? What does it describe?

i)      What is KEGG? What information is used?

j)     What are major problems with transferring functional annotations form one to another organism?

k)    Why is a functional annotation of more than 2/3 of the genes in a plant genome highly unlikely?

l)      What is a problem by using sequence similarity for annotations?

Plant genome and transcriptome annotations, from misconceptions to simple solutions, Bolger et al., 2017.

A beginner's guide to eukaryotic genome annotation, Yandel et al., 2012.

Extra:

The NCBI Eukaryotic Genome Annotation Pipeline: https://www.ncbi.nlm.nih.gov/genome/annotation_euk/process/