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A complicating factor is recombination between SNPs and QTLs. This means that the value of animals in the reference population breaks down when the number of generations between them and the test population increases (more chance for recombination events). And it implies that it is highly recommended to continue the recording of phenotypic data of future generations. The best policy is to extend the reference population with DNA- and phenotypic data from new generations while data from the oldest generations can be deleted, but the reference population should always have a sufficient (large) size.
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